HelioBLAST
HelioBLAST is a web-based tool for comparing protein or nucleotide sequences to sequence databases. It allows users to do quick similarity searches to identify potential homologs or characterize new sequences.
sequence-alignment
homology-detection
web-tool
HelioBLAST: Protein Sequence Comparison Tool
HelioBLAST is a web-based tool for comparing protein or nucleotide sequences to sequence databases. It allows users to do quick similarity searches to identify potential homologs or characterize new sequences.
What is HelioBLAST?
HelioBLAST is a web-based tool developed by the National Center for Biotechnology Information (NCBI) for comparing protein or nucleotide sequences to sequence databases like GenBank. It functions similarly to BLAST, allowing users to do quick similarity searches to identify potential homologs or functionally characterize new sequences.
Some key features of HelioBLAST include:
- Intuitive web interface for pasting in query sequences and choosing database parameters
- BLAST+ algorithms for fast sequence alignment and comparison
- Access to the full NCBI sequence databases with billions of bases
- Graphical output with sequence alignments, e-values, and links to related database entries
- Options to customize parameters like word size, match/mismatch scores, and e-value threshold
- Integration with NCBI tools like CDD for conserved domain detection
Overall, HelioBLAST allows molecular biologists, bioinformaticians, and other researchers to quickly search and compare novel protein or nucleotide sequences to identify homologs, characterize domains/motifs, or infer sequence function. The web interface and powerful backend algorithms facilitate both basic similarity searches during early investigation as well as optimized, customized searches.
HelioBLAST Features
Features
- BLAST search against custom databases
- Support for protein and nucleotide databases
- Adjustable parameters like E-value threshold
- Visualization of BLAST hits
- Sequence alignment viewer
- Downloadable results in various formats
Pricing
- Free
- Open Source
Pros
Easy to use web interface
Fast searches against large databases
Free and open source
Custom database support
Visualizations help interpret results
Cons
Limited to BLAST algorithm
Less customizable than standalone BLAST
Requires uploading sequences rather than pasting
Only provides web interface, no APIs
Official Links
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