DNAssist icon

DNAssist

DNAssist is open-source software used for DNA sequence design and analysis. It allows scientists to easily design primers, probes, and cloning strategies for PCR, sequencing, and molecular cloning experiments.

What is DNAssist?

DNAssist is an open-source, cross-platform software tool used for DNA sequence design and analysis. It was developed at the National Institute of Standards and Technology (NIST) to help scientists easily design primers, probes, and cloning strategies for common molecular biology experiments like PCR, sequencing, and cloning.

Some key features of DNAssist include:

  • Intuitive graphical user interface for visualizing DNA sequences
  • Tools for finding and avoiding primer dimers or hairpins
  • PCR primer and probe design with Tm and GC content calculations
  • Plasmid maps and sequence analysis tools
  • Support for designing Gibson assembly and golden gate cloning strategies
  • Ability to batch process large numbers of sequences
  • Export of publication-ready vector maps and sequence files

DNAssist runs on Windows, Mac OS X, and Linux systems. The software and full documentation are available for free download under an open-source license from the NIST website. It provides scientists with a user-friendly desktop application for handling routine DNA sequence manipulations required in modern molecular biology labs.

The Best DNAssist Alternatives

Top Apps like DNAssist

SnapGene Viewer, Chromas, BioEdit, Geneious, DNADynamo, ApE - A plasmid Editor, PlasmaDNA, Open Vector Editor Electron, GeneStudio, Gene Construction Kit are some alternatives to DNAssist.

SnapGene Viewer

SnapGene Viewer is a free, standalone software application developed by GSL Biotech for visualizing and analyzing DNA sequences and genetic maps. It supports an extensive range of molecular biology file formats including GenBank, FASTA, ABI trace files, Vector NTI, DNA Star, ApE, and SnapGene files.Key features of SnapGene Viewer...

Chromas

Chromas is a free, open-source spectral analyzer plugin released in 2017. It is lightweight software designed to work as an audio effect plugin in digital audio workstations like Ableton Live, Logic Pro, FL Studio, Reaper, and more.With Chromas added to an audio track, it displays a colorful graphical representation of...

BioEdit

BioEdit is a versatile biological sequence alignment editor and analysis software for Windows. First released in 1997 by Tom Hall, BioEdit has become one of the most widely used bioinformatics tools for molecular biology research.Key features of BioEdit include:User-friendly graphical interface for editing and analyzing DNA, RNA, and protein...

Geneious

Geneious is a commercial bioinformatics software platform developed by Biomatters for DNA, RNA, and protein sequence analysis and manipulation. It provides an intuitive graphical user interface and integrates multiple commonly used tools for genomics research.Some key features of Geneious include:Sequence alignment with both global and local algorithmsPhylogenetic tree...

DNADynamo

DNADynamo is a powerful DNA sequence design and optimization software tool developed by Blue Heron Biotech. It enables synthetic biologists and biological engineers to design novel DNA components and pathways such as promoters, transcription factors, genes, vectors, and more.Some key features of DNADynamo include:Intuitive drag-and-drop graphical user interface...

ApE - A plasmid Editor

ApE (A Plasmid Editor) is a feature-rich software application for creating, viewing, editing, graphing, and analyzing DNA plasmid sequences. It is designed to be simple to use, yet powerful enough for the daily tasks of a molecular biologist.Some key features and capabilities of ApE include:Intuitive graphical user interface...

PlasmaDNA

PlasmaDNA is an advanced data lineage and metadata platform designed to help organizations better understand and govern their data assets. It automatically reverse engineers and maps out technical, business and operational metadata to create a searchable knowledge graph of all data flows and processes.Key capabilities include:Automated discovery of...

Open Vector Editor Electron

Open Vector Editor Electron is a free, open source vector graphics editor for Windows, Mac and Linux operating systems. It is built using the Electron framework which allows creating cross-platform desktop applications using web technologies.The editor allows users to create and edit SVG vector images and icons. It has...

GeneStudio

GeneStudio is a free, open-source desktop application for molecular biology and genetics research. It provides a wide range of tools for DNA, RNA, and protein sequence analysis, including sequence alignment, motif finding, secondary structure prediction, phylogenetic tree building, 3D molecular modeling, and more.Some key features of GeneStudio include:Sequence...

Gene Construction Kit

Gene Construction Kit (GCK) is an open-source software tool designed for researchers and engineers working in synthetic biology and genetic engineering. It provides an integrated platform for designing, visualizing, and analyzing novel DNA sequences prior to physical construction.Some key features of GCK include:User-friendly graphical interface for combining basic...